Cresol Red
Appearance
(Redirected from Cresol red)
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Names | |
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Preferred IUPAC name
3,3-Bis(4-hydroxy-3-methylphenyl)-2,1λ6-benzoxathiole-1,1(3H)-dione | |
Identifiers | |
3D model (JSmol)
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ChEBI | |
ChemSpider | |
ECHA InfoCard | 100.015.513 |
PubChem CID
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UNII | |
CompTox Dashboard (EPA)
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Properties | |
C21H17NaO5S | |
Molar mass | 382.43 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Cresol red (full name: o-cresolsulfonephthalein)[1] is a triarylmethane dye frequently used for monitoring the pH in aquaria.
Cresol red (pH indicator) | ||
below pH 7.2 | above pH 8.8 | |
7.2 | ⇌ | 8.8 |
Molecular biology
[edit]Cresol red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.
Color marker
[edit]Cresol red can also be used as an electrophoretic color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel, it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.
References
[edit]- ^ PubChem. "Cresol red". pubchem.ncbi.nlm.nih.gov. Retrieved 2023-01-25.