Optical darkfield imaging in microscopy involves forming images using a back focal-plane (scattering angle) aperture that excludes the unscattered beam. It's called "dark field" because the field surrounding the specimen doesn't scatter, so it's dark. This digital darkfield animation illustrates by placing an aperture (centered in the orange figure at left) over the power spectrum (a digital substitute for the back focal-plane's optical diffraction pattern) shown with the DC peak (or unscattered beam) below center. In this example of 2nm metal particles distributed on a nano-cylinder, only nanocystals with projected periodicities that diffract into the aperture light up in the darkfield image at right and this varies with aperture position. The aperture is moving by 1.25 degree increments around the ring associated with diffraction from gold 2.3 Ångstrom (111) lattice spacings.
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P. Fraundorf
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{{Information |Description=Optical darkfield imaging in microscopy involves forming images using a back focal-plane (scattering angle) aperture that excludes the unscattered beam. It's called "dark field" because the field surrounding the specimen doesn't